Immunohistochemical indices of some types of hormones among benign tumors of the salivary glands
Summary. Immunohistochemistry (IHC) in the diagnosis of salivary glands (SG) tumors remains the gold standard for final verification of tumors, along with pathomorphological examination of the resectatum. The most prominent markers are various types of human papillomavirus, cytomegaloviruses, Epstein — Barr virus, progesterone (PR), estrogen (ER), androgen (AR) receptors, various cytokeratins to confirm tumors malignancy. The majority of recent works devoted to presence and possibility of endocrine regulation influence on SG tumors tissues testify to presence of PR, ER, AR. Their amount in SG tumors tissues is quite diverse and depends on geographical population. Objective — to study the amount of PR, ER in SG tumors tissues by IHC. Materials and methods. Histologic typing of SG tumors was performed using routine coloration with hematoxylin and eosin and IHC. The study was conducted based on excisional biopsy of SG (pleomorphic adenoma and adenolymphomas) in 32 patients. IHC was performed with Monoclonal Rabbit antibody Anti-Human ER («Dako IS 151», Denmark), Monoclonal Mouse antibody Anti-Human PR («Dako IR068», Denmark) using «EnVisionTM FLEX Detection System» («Dako», Denmark). For positive control tissue samples with definite positive reactivity were used, for negative — the procedure was performed without the use of primary antibodies. Results. Data analysis showed that all patients with pleomorphic adenomas had only parotid SG affected. Among the hormones present in the tissues of parotid SG tumor 3 (10.7%) is progesterone, 2 (7.14%) is estrogen. Hormones indicators of estrogen dominated among SG adenolymphomas — 3 (75%). Conclusions. SG benign tumors (pleomorphic adenomas and adenolymphomas) are characteristic of ER and PR expression. Tumors localized in parotid SG in all patients demonstrating positive reactions to expression of ER and PR.
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