Украинский Медицинский Журнал
Издательство МОРИОН АПТЕКА online Компендиум Клиническая онкология Работа в медицине и фармации
Menu
  • Home
  • Online
  • Last issue
  • Archive
  • About the Journal
  • Instructions for authors
  • Authors
  • Organizations
  • Categories
    Back
    • Case report in clinical practice
    • Distance education
    • Events and comments
    • For a medical practitioner
    • History of medicine
    • Medical ethics
    • Medical law
    • Research article
    • Review
    • Scientific discussions
    • Standards and protocols
    • Topical issue
  • Specialties
    Back
    • Allergology and Pulmonology
    • Anesthesiology and Intensive care
    • Cardiology, Rheumatology
    • Dermatology and Venereology
    • Endocrinology
    • Gastroenterology
    • Genetic and Regenerative medicine
    • Healthcare organization
    • Immunology
    • Infectious diseases
    • Medical emergency
    • Medical law
    • Neonatology
    • Nephrology and Urology
    • Neurology and Psychiatry
    • Obstetrics and Gynecology
    • Oncology, Hematology
    • Ophthalmology
    • Orthopedics and Traumatology
    • Otorhinolaryngology
    • Pediatrics
    • Pharmacoeconomics
    • Pharmacology
    • Phthisiology
    • Preventive medicine
    • Radiology
    • Scientific discussions
    • Stomatology
    • Surgery
    • Therapy, General practice
  • English
  • Рус/Укр
Украинский Медицинский Журнал
Знайти...
  • Categories
  • Specialties
  • Case report in clinical practice
  • Distance education
  • Events and comments
  • For a medical practitioner
  • History of medicine
  • Medical ethics
  • Medical law
  • Research article
  • Review
  • Scientific discussions
  • Standards and protocols
  • Topical issue
  • Allergology and Pulmonology
  • Anesthesiology and Intensive care
  • Cardiology, Rheumatology
  • Dermatology and Venereology
  • Endocrinology
  • Gastroenterology
  • Genetic and Regenerative medicine
  • Healthcare organization
  • Immunology
  • Infectious diseases
  • Medical emergency
  • Medical law
  • Neonatology
  • Nephrology and Urology
  • Neurology and Psychiatry
  • Obstetrics and Gynecology
  • Oncology, Hematology
  • Ophthalmology
  • Orthopedics and Traumatology
  • Otorhinolaryngology
  • Pediatrics
  • Pharmacoeconomics
  • Pharmacology
  • Phthisiology
  • Preventive medicine
  • Radiology
  • Scientific discussions
  • Stomatology
  • Surgery
  • Therapy, General practice
Advertising in the issue 1-2 (147-148) – I/IV 2022
  • All electronic publications
3 (131) – V/VI 2019 :
  • Research article

Immunohistochemical indices of some types of viruses among benign tumors of salivary glands

Malanchuk V.O.1, Brodetskyi I.S.1, Krotevych M.S.2

  • 1National Medical University named after A.A. Bogomolets, Kyiv
  • 2National Cancer Institute, Kyiv

Summary. The purpose — identification of the etiological factors of deve­lopment of benign tumors of the salivary glands (SG) among certain groups of viruses. Materials and methods. Histological typing of SG tumors was performed using coloration with hematoxylin and eosin and immunohistochemical analysis. The study was conducted based on excision biopsies of SG (pleomorphic adenomas, adenolymphomas) in 32 patients. Immunohistochemical analysis was performed with Monoclonal mouse anti-Epstein — Barr virus, LMP, clones CS. 1–4 («Dako» IS75330-2, Denmark), Polyclonal rabbit antibody p16 (CDKN2A) («Thermo scientific» PAL. 16662) using the «EnVisionTM FLEX System». For positive control tissue samples with definite positive reactivity were used, for negative — the procedure without the use of primary antibodies was performed. Results. The majo­rity of patients with pleomorphic adenomas were affected in parotid SG. Among viruses that were presented in the tumor tissues of parotid SG in 19 (67.8%) patients the HPV 16 and Epstein — Barr virus (EBV) — 8 (28.6%) were dominated. Among SG adenolymphomas the HPV 16 dominated — 4 (100.0%). Conclusions. In majority of patients who have been detected positive reactions to receptors expression against HPV 16 and EBV neoplasms were localized in parotid SG. For the most part of positive reactions among pleomorphic adenomas and adenolymphomas of SG to HPV 16 were 21 (75.0%) and 4 (100.0%) respectively.

DOI 10.32471/umj.1680-3051.131.158532

Published: 18.06.2019

References:

  • Malanchuk V.O. (red.) (2011a) Khirurhichna stomatolohiia ta shchelepno-lytseva khirurhiia. 1, Lohos, Kyiv, 627 s.
  • Malanchuk V.O. (red.) (2011b) Khirurhichna stomatolohiia ta shchelepno-lytseva khirurhiia. 2, Lohos, Kyiv, 606 c.
  • Paches A.I. (red.) (2009) Opuholi slyunnyih zhelez: Prakt. meditsina, Moskva, 470 s.
  • Chen J., Foroozesh M., Qin Z. (2019) Transactivation of human endogenous retroviruses by tumor viruses and their functions in virus-associated malignancies. Oncogenesis, 8(1): 6.
  • Chen L.P., Thomas E.K., Hu S.L. et al. (1991) Human papillomavirus type 16 nucleoprotein E7 is a tumor rejection antigen. Proc. Natl. Acad. Sei. USA, 88(1): 110–114.
  • Da Silva L.P., Serpa M.S., Viveiros S.K. et al. (2018) Salivary gland tumors in a Brazilian population: a 20-year retrospective and multicentric study of 2292 cases. J. Craniomaxillofac. Surg., 46(12): 2227–2233.
  • Hühns M., Simm G., Erbersdobler A., Zimpfer A. (2015) HPV infection, but not EBV or HHV-8 infection, is associated with salivary gland tumours. BioMed. Res. Int., 1–7.
  • Laane C.J., Murr A.H., Mhatre A.N. et al. (2002) Role of Epstein — Barr virus and cytomegalovirus in the etiology of benign parotid tumors. Head Neck, 24(5): 443–450.
  • Lin F.C.F., Chen P.L., Tsao T.Y. et al. (2014) Prevalence of human papillomavirus and Epstein — Barr virus in salivary gland diseases. J. Int. Med. Res., 42(5): 1093–1101.
  • Miah M.S., Majumdar S., White S. et al. (2015) Human papillomavirus and salivary gland neoplasia: a p16INK4 immunohistochemical and in situ hybridisation study. J. Laryngol. Otol., 129(10): 1000–1003.
  • Müller-Coan B.G., Caetano B.F.R., Pagano J.S., Oliveira E.D.E. (2018) Cancer progression goes viral: the role of oncoviruses in aggressiveness of malignancies. Trends Cancer, 4(7): 485–498.
  • Prichard J.W. (2014) Overview of automated immunohistochemistry. Arch. Pathol. Lab. Med.,138(12): 1578–1582.
  • Skálová A., Kašpírková J., Andrle P. et al. (2013) Human papillomaviruses are not involved in the etiopathogenesis of salivary gland tumors. Cesk. Patol., 49(2): 72–75.
Comments
No Comments » Add own
Leave a comment

If you notice an error select the text with error and press Ctrl + Enter to tell as about it
Full article in pdf format
Keywords:
adenolymphoma, Epstein — Barr virus, human papillomavirus type 16, immunohistochemistry analysis, pleomorphic adenoma, tumors of salivary glands
Specialties:
Immunology, Oncology, Hematology, Stomatology, Surgery
Views: 21
Украинский медицинский журнал

«Morion» publishing house
Editorial office and publisher: 02140, Kyiv, ave. Bazhana 10A
Tel.: +380 (44) 585-97-10

Specialized medical online edition for doctors,
pharmacists, students of medical and pharmaceutical universities
Disclaimer

Издательство МОРИОНmorion.ua АПТЕКА onlineapteka.ua Компендиум — справочник лекарств №1compendium.com.ua