Immunohistochemical indices of some types of viruses among benign tumors of salivary glands
Malanchuk V.O.1, Brodetskyi I.S.1, Krotevych M.S.2
Summary. The purpose — identification of the etiological factors of development of benign tumors of the salivary glands (SG) among certain groups of viruses. Materials and methods. Histological typing of SG tumors was performed using coloration with hematoxylin and eosin and immunohistochemical analysis. The study was conducted based on excision biopsies of SG (pleomorphic adenomas, adenolymphomas) in 32 patients. Immunohistochemical analysis was performed with Monoclonal mouse anti-Epstein — Barr virus, LMP, clones CS. 1–4 («Dako» IS75330-2, Denmark), Polyclonal rabbit antibody p16 (CDKN2A) («Thermo scientific» PAL. 16662) using the «EnVisionTM FLEX System». For positive control tissue samples with definite positive reactivity were used, for negative — the procedure without the use of primary antibodies was performed. Results. The majority of patients with pleomorphic adenomas were affected in parotid SG. Among viruses that were presented in the tumor tissues of parotid SG in 19 (67.8%) patients the HPV 16 and Epstein — Barr virus (EBV) — 8 (28.6%) were dominated. Among SG adenolymphomas the HPV 16 dominated — 4 (100.0%). Conclusions. In majority of patients who have been detected positive reactions to receptors expression against HPV 16 and EBV neoplasms were localized in parotid SG. For the most part of positive reactions among pleomorphic adenomas and adenolymphomas of SG to HPV 16 were 21 (75.0%) and 4 (100.0%) respectively.
DOI 10.32471/umj.1680-3051.131.158532
Published: 18.06.2019
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